Summary
Massively parallel methods that read millions of DNA fragments simultaneously, cutting cost and time.
In depth
Library preparation, amplification, parallel sequencing and bioinformatics assembly. Throughput increased about a million-fold since Sanger. Applications: whole genome, exome, RNA-seq, ChIP-seq and metagenomics.
Library prep fragments DNA and adds adapters. Sequencing by synthesis detects fluorescent nucleotides as they are added. Depth and read length depend on the application.
Example: exome sequencing targets about 1 to 2 percent of the genome for rare disease diagnosis.
Frequently asked questions
What is Next-Generation Sequencing?
Massively parallel methods that read millions of DNA fragments simultaneously, cutting cost and time.
Which area of biology covers Next-Generation Sequencing, and how advanced is it?
Next-Generation Sequencing is one of the 56 topics in Biotechnology & Genomics. It is rated Advanced, so it suits learners who want specialist depth. Related topics in this area include DNA Sequencing, Polymerase Chain Reaction, Human Genome Project.
Can you give an example of Next-Generation Sequencing?
Newborn genome sequencing can detect hundreds of conditions.
What should I learn before Next-Generation Sequencing?
Work through the Beginner topics in Biotechnology & Genomics first, such as Biotechnology, Genetic Engineering, Selective Breeding, then return to Next-Generation Sequencing. Advanced topics assume the Intermediate vocabulary.
What topics are related to Next-Generation Sequencing?
The closest topics to Next-Generation Sequencing are DNA Sequencing, Polymerase Chain Reaction, Human Genome Project, Functional Genomics. Comparing them side by side is a good way to see how the ideas connect.
How can I check that I understand Next-Generation Sequencing?
Read the summary and the in-depth section, then use the quick check on the interactive lesson for Next-Generation Sequencing. To practise the whole of Biotechnology & Genomics, use the Biotechnology & Genomics quiz.
How is Next-Generation Sequencing different from DNA Sequencing?
Next-Generation Sequencing: Massively parallel methods that read millions of DNA fragments simultaneously, cutting cost and time. DNA Sequencing: Determining the order of bases, from Sanger chain termination to modern high-throughput methods. Read both side by side and note what each one explains.
How should I study Next-Generation Sequencing step by step?
First, read the summary and write the definition in your own words. Second, read the in-depth section and note any example. Third, compare it with DNA Sequencing and explain the difference aloud. Finally, take the Biotechnology & Genomics quiz and come back to this page for any question you miss.