Measures the colour intensity of a solution to estimate concentration.
Colorimeters measure absorbance at a chosen wavelength using filters. A calibration curve from standards allows concentration from absorbance. Used in enzyme assays, such as the Benedict's or Bradford test.
Zero the instrument with a blank. Choose a complementary colour filter. Standards of known concentration give the calibration curve.
Key idea: more concentrated coloured solutions absorb more light.
Measures the colour intensity of a solution to estimate concentration.
Colorimetry is one of the 51 topics in Lab Methods & Biostatistics. It is rated Intermediate, so it suits learners who already know the core vocabulary. Related topics in this area include Spectrophotometry, Variables, ELISA.
Measuring starch breakdown with iodine.
Work through the Beginner topics in Lab Methods & Biostatistics first, such as Scientific Method, Hypothesis, Variables, then return to Colorimetry. Intermediate topics assume the Beginner vocabulary.
The closest topics to Colorimetry are Spectrophotometry, Variables, ELISA, Cell Fractionation. Comparing them side by side is a good way to see how the ideas connect.
Read the summary and the in-depth section, then use the quick check on the interactive lesson for Colorimetry. To practise the whole of Lab Methods & Biostatistics, use the Lab Methods & Biostatistics quiz.
Colorimetry: Measures the colour intensity of a solution to estimate concentration. Spectrophotometry: Determines how much light of a given wavelength a sample absorbs, linked to concentration by the Beer-Lambert law. Read both side by side and note what each one explains.
First, read the summary and write the definition in your own words. Second, read the in-depth section and note any example. Third, compare it with Spectrophotometry and explain the difference aloud. Finally, take the Lab Methods & Biostatistics quiz and come back to this page for any question you miss.
Determines how much light of a given wavelength a sample absorbs, linked to concentration by the Beer-Lambert law.
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