Summary
DNA made by combining sequences from different sources, typically using restriction enzymes and ligase.
In depth
Paul Berg made the first recombinant molecule in 1972; Cohen and Boyer created recombinant plasmids in bacteria in 1973. Combination allows the production of foreign proteins in host cells.
The recombinant plasmid replicates with the host, producing many copies of the insert. Selecting transformed cells uses antibiotic resistance genes. Safety guidelines restrict use of dangerous genes.
Example: recombinant hepatitis B vaccine antigen is made in yeast.
Frequently asked questions
What is Recombinant DNA?
DNA made by combining sequences from different sources, typically using restriction enzymes and ligase.
Which area of biology covers Recombinant DNA, and how advanced is it?
Recombinant DNA is one of the 56 topics in Biotechnology & Genomics. It is rated Intermediate, so it suits learners who already know the core vocabulary. Related topics in this area include Insulin Production, Transgenic Organisms, Vaccines by Genetic Engineering.
Can you give an example of Recombinant DNA?
Recombinant human growth hormone replaced cadaver-derived hormone, which carried a prion risk.
What should I learn before Recombinant DNA?
Work through the Beginner topics in Biotechnology & Genomics first, such as Biotechnology, Genetic Engineering, Selective Breeding, then return to Recombinant DNA. Intermediate topics assume the Beginner vocabulary.
What topics are related to Recombinant DNA?
The closest topics to Recombinant DNA are Insulin Production, Transgenic Organisms, Vaccines by Genetic Engineering, Genetic Engineering. Comparing them side by side is a good way to see how the ideas connect.
How can I check that I understand Recombinant DNA?
Read the summary and the in-depth section, then use the quick check on the interactive lesson for Recombinant DNA. To practise the whole of Biotechnology & Genomics, use the Biotechnology & Genomics quiz.
How is Recombinant DNA different from Insulin Production?
Recombinant DNA: DNA made by combining sequences from different sources, typically using restriction enzymes and ligase. Insulin Production: Human insulin is made by bacteria carrying the human gene, replacing animal sources. Read both side by side and note what each one explains.
How should I study Recombinant DNA step by step?
First, read the summary and write the definition in your own words. Second, read the in-depth section and note any example. Third, compare it with Insulin Production and explain the difference aloud. Finally, take the Biotechnology & Genomics quiz and come back to this page for any question you miss.